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Mismatches and high concentrations of ATP drive accumulation of adenylated intermediates, with only slow, partial conversion to ligated product, likely limited by enzyme re-adenylation.
Both sequences were sufficient to drive accumulation of EGFP in the nucleus, with stronger nuclear targeting observed for EGFP-NLS2.
The DEAKTIV analogue was chosen as a control because it lacks the fixed positive charge needed to drive accumulation into mitochondria.
If the effect of TIGAR knockdown is to drive accumulation of vesicles through inhibiting their maturation, we would expect bafilomycin A1 treatment to neutralize the effect of TIGAR.
Our findings suggest that the novel SR-A ligands, which are found as insoluble deposits at sites of chronic inflammation, contribute to retention of macrophages and induce a pro-inflammatory, oxidative state, which in turn would drive accumulation of pro-atherogenic oxidized lipoproteins in the arterial wall or induce neurological damage in the brain.
In prokaryotes, ABC importers drive accumulation of nutrients in the cytoplasm against their concentration gradients while ABC exporters remove toxic substrates out of the cytoplasm and may function as flippases of lipids (Higgins and Linton, 2004; Rees et al., 2009; Sharom, 2011; George and Jones, 2012).
Similar(53)
Ethylene drives accumulation of SUB1A mRNA.
The loss of Zap70 catalytic activity does not affect the integrin driven accumulation of actin at the synapse, but results in loss of actin reorganisation once the synapse has formed.
The increased retention of MPs also controlled release and drove accumulation of polyIC within the LN and in LN-resident APCs, resulting in more enduring activation of DCs (Fig. 6b) (53).
Another process that drives accumulation of STIM1 into ER PM junctions is an increase in temperature [ 24]; under such conditions the depletion of the ER Ca2+ stores is not required to reveal the junctions.
We have made a number of changes to clarify the evidence that the Anaef mutation has 2 distinct effects: (1) it increases naïve T-cell CD44 expression and (2) it drives accumulation of CD44hi effector CD4+ T cells and autoantibodies.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com