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A drug intake of approximately 30 mg/kg/day was achieved by adapting the concentrations of the NK1 receptor antagonist in the drinking solutions according to mean drinking volume and body weight per cage.
Lead drinking solutions were provided to dams 2 weeks before mating to ensure BPb concentration stabilization and a Pb body burden, throughout gestation and until PND10.
Lead drinking solutions were provided to dams 2 weeks prior to mating to ensure [BPb] stabilization and a lead body burden throughout gestation and until PN10.
For this reason, adult female rats (n = 8 9/group) were treated for 30 days with drinking solutions containing 0.2, 0.5, or 1.0 ppm THF-diols in 2% Tween 80 vehicle.
Figure 2 is a schematic of the cyclicity data collected from the vehicle controls and the 0.2-, 0.5-, and 1.0-ppm THF-diol treatment groups administered the compounds for 30 days as drinking solutions.
Drinking solutions containing 2 ppm LTX-diols completely blocked cyclicity in female rats, even though they failed to affect male sexual behavior (Mani et al. 2005; Markaverich et al. 2005).
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HAL was infused via osmotic pumps (0.1 mg/kg/day) during a 14-day regimen of chronic caffeine administered in a caffeinated drinking solution (≈136 mg/kg/day).
In ovo injection of AgNano at early embryonic stages of the development can enhance the effect of AgNano, distributed as a drinking solution, on FGF2 and VEGFA expression.
However, the AgNano drinking solution itself (both concentrations) evoked up-regulation of FGF2 expression in the muscle, without enhancing the effect of in ovo injection.
Answering this question, we assumed that indeed, in ovo injection up-regulated both the heart expression of FGF2 as well as the muscle expression of VEGFA in broilers treated with drinking solution of 20 ppm of AgNano.
Red arrows indicate random selection of the birds after hatching, from the in ovo part (eggs not injected and injected with AgNano) to the in vivo part (broilers treated with AgNano drinking solution) of the experiment.
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