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The principles of the drift correction algorithms that automatically correct the vertical inaccuracy are presented and the performance of the algorithms is evaluated.
Scan start points were detected and marked (using average gradient and criterion for continuous scans) and corrected (using template drift correction).
Coarse drift correction was performed using the Correct 3D Drift plugin in FIJI (Parslow et al., 2014).
Microscope drift during acquisition can be corrected using the 3D drift correction plugins, either by autocorrelation analysis or using fiducial markers.
Diaphragm drift due to irregular respiration was corrected automatically by the diaphragm drift correction system provided by the MR system.
For gel source data, see Supplementary Fig. 1. b, Representative micrograph of the sample after drift correction and dose weighing.
Wang, Y. et al. Localization events-based sample drift correction for localization microscopy with redundant cross-correlation algorithm.
We minimize the total energy function synchronously for template tracking and weighted active drift correction.
Drift correction and the calibration method for far-field measurements are explained.
In this paper, we propose a novel algorithm for object template tracking and its drift correction.
In our algorithm, the total energy function consists of two terms: the tracking term and the drift correction term.
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