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Organic phase was collected in a glass tube and dried under stream of nitrogen.
The final extract was dried under stream of nitrogen, dissolved in HPLC grade acetonitrile (ACN THF (8 2, v:v) (Sigma) mixture and injected into an Agilent 1100 HPLC system employing a reverse phase Zorbax Eclipse XDB-C18 column (250 mm × 4.6 mm).
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The hexane phases were transferred into a 1.5 ml tube and dried under streaming nitrogen.
The hexane phase was dried under streaming nitrogen and redissolved with 50 μl chloroform.
The respective HPLC fractions were dried under streaming nitrogen, subsequently resuspended in 10 μL of acetonitrile/H2O/acetic acid (90 10 0.1, by vol).
Samples were dried under streaming nitrogen and 200 μl 2-alkenyl-4, 4-dimethyloxazoline (Sigma, München) were added.
After vigorous shaking and a brief centrifugation at 4 °C for 10 min the upper layer was subsequently taken and dried under streaming nitrogen.
The extracts were combined, dried under a stream of nitrogen, and freeze dried to remove residual water.
The wafer was then dried under a stream of nitrogen.
Then, it was dried under a stream of nitrogen gas.
The extracts were dried under a stream of nitrogen at a temperature below 40 °C.
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