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The pin was rinsed with sonication and dried extensively between sample switching.
The solution was dried extensively to remove chloroform.
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After removing the crystal violet solution, the plates were washed extensively, dried, and the stain released using 2% SDS in PBS.
Cells were plated on coverslips previously etched with 70% nitric acid and rinsed over 2 3 days, then coated with poly-L-lysine-coated (1 mg/ml in 0.1 M sodium borate buffer, pH 8.5) overnight, dried and washed extensively.
The reaction mixture was refluxed for 6 h, then evaporated to dryness, and extensively dried under high vacuum.
All samples were then simultaneously analyzed on denaturing SDS PAGE gels and extensively dried, and the γ-P-labeled CheY band was quantified using phosphorimaging.
The TiO2-NRA substrate was removed, rinsed extensively with deionized water, and dried under airflow.
Briefly, glass coverslips were sonicated, rinsed extensively with hot water and dried in a N2 stream.
A white powder was recovered after filtration through a por.3 Gooch, extensively washed with ether and dried under vacuum and stored in a dessicator over KOH pellets.
Perlite was pretreated with 1.2 M HNO3 at 60°C for 4 hours and then extensively washed with water and dried at 60°C.
The as-obtained PS particles with a diameter at about 450 nm were washed extensively with ethanol in centrifuge and dried in air at room temperature.
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