Immunoglobulin was isolated using an affinity chromatography system (AKTA explore, GE, USA) and freeze dried (Rlphr 1 4 LSC freeze drier, Christ, German).
The wet cell mass was frozen overnight at −70°C and then lyophilized in a freeze drier (Christ Alpha 1 2 LD Plus, Germany) to measure cell dry weight and total lipid content.
The extracted peptides were dried on SpeedVac (Christ) and subsequently dissolved in 5 μl 0.1% FA in 50% AcN.
Protein spots excised from SYPRO Ruby-stained gels were destained with 100 m M ammonium bicarbonate in 50% acetonitrile (AcN; Sigma-Aldrich), and then washed with 100% AcN and dried on SpeedVac (Christ, Osterode am Harz, Germany).
The sediments were freeze-dried (Christ alpha 1-4LD, Germany) before the oil analysis and 20 g of sediment samples were extracted with a mixture of n-hexane and dichloromethane.
Elution was performed by using the same ÄKTA purifier system, at flow rate of 1 mL/min, in 1.5 column volumes; the chromatographic profiles were detected at 215 nm, the eluted peaks were collected and then freeze-dried (Christ Epsilon 2-6D, Martin Christ, Germany).
The fresh fruiting bodies were sliced, weighed and freeze-dried (Christ, Germany) for 1 2 days.
Scalpel dissected root fragments (1 cm long) of mycorrhizal (21 dpi) and non-mycorrhizal Medicago truncatula plants were snap-frozen in liquid N2and thoroughly dried by 12 h lyophilisation (ALPHA 2 4, Christ, Osterode, Germany) to inhibit metabolite changes due to enzyme activity and the loss of solutes.
The collected yeast cells were washed once with distilled water and dried using a freezer dryer (Alpha 2 4 LSC, Christ GmbH).
Dialyzed and concentrated urine was subsequently snap frozen in a bath of liquid nitrogen and dried in a Beta 1 8 LD® freeze dryer (Christ, Osterode, Germany).
Mary of Bethany may have used a jar when she repented of her sins at Christ's feet in her home; by the Renaissance, the image of the Magdalen was of the woman who bathed Christ's feet with her tears and dried them with her hair.
