This analysis resulted in a repertoire of 146 DRB, 74 DQB, 58 DQA, 45 DPB, and 19 DPA eplets.
DRB (5,6-dichlorobenzimidazone-1-β-D-ribofuranoside) is a commonly used reagent for Cdk9 inhibition in cell biology studies.
Patch determinations with a molecular viewer of crystalline structural models downloaded from the Entrez Molecular Modeling Database Web site led to the identification of 44 DRB, 33DQB, 29 DQA, 20 DPB, and 9 DPA unique combinations of polymorphic positions.
McCormick ME, Kraemer DRB (2006).
A closed reflux digester of HACH, USA (model: DRB 200) was used for digestion.
The COD was determined according to Standard Methods (Eaton et al. 2005), using a spectrophotometer DRB 200 (Hach Company, Loveland, CO).
SLA class II lacks DPA1, DPB1, DRB3, DRB4, and DRB 5 in humans.
CHO cells were treated with DRB (50 µM), ActD (0.05 µg/ml), or DRB+ActD for 30 minutes, and then uridine incorporation was analyzed (Fig. 5B).
At such time, previous studies have shown that mammalian cells become insensitive to inhibitors of RNA polymerase II, such as α-amanitin or 5,6-dichloro-ribofuranosyl-benzimidazole (DRB) [28], [29], [30].
This was shown by determining the times in G1 when cells become insensitive to two potent and specific inhibitors of RNA polymerase II function: α-amanitin or 5,6-dichloro-ribofuranosyl-benzimidazole (DRB) [35], [36].
