Peripheral blood (PB) was drawn in PAXgene RNA isolation tubes (PreAnalytix) from 18 patients and 15 controls.
Blood (2.5 mL) was drawn in PAXgene blood RNA isolation tubes (PreAnalytiX GmbH) and stored at -20°C.
Samples for cell isolation and flow cytometry were drawn in EDTA tubes.
For RNA isolation, 2.5 ml blood was drawn in PAXgene tubes, incubated for two hours at room temperature and stored at -20°C.
However, this pressurization strategy could increase the risk of isolation patients acquiring secondary infections from contaminated air drawn in from adjacent spaces.
Fresh venous blood was drawn in 10 ml EDTA tubes from patients and controls and processed in parallel continuously from PBMC isolation to cytokine measurement.
The blood was drawn in a sterile fashion, centrifuged at 200 ×g for the supernatant, then 1500 ×g for platelet isolation.
Blood was drawn in the morning after an overnight fast from hyperlipidemic patients and HC for the determination of biochemical and hematological parameters and for PMNL isolation.
Even a draw, while in isolation a very good result, is not really enough for them.
Eight millilitres of blood were drawn for the isolation of PBMCs in a vaccutainer CPT tube (Beckton Dickinson Diagnostics, Franklin Lakes, NJ).
