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To estimate the volume of each layer, camera lucida drawings of sections (6 8 sections per animal, 300 µm inter-section intervals) through the entire MOB (from the anterior MOB to the anterior AOB) were made from low-magnification photographs.
The retrogradely labeled cells, forming thalamic territories projecting to the different cortical areas injected, were each represented by a symbol specific for each tracer on serial drawings of sections of the thalamus (Figs 1 and 2), where nuclear borders were delineated on the basis of Nissl and acetylcholinesterase stainings (see Supplementary Materials and Methods).
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Sections were inspected through a microscope and the areas of damage plotted on drawings of brain sections from a rhesus monkey brain atlas (Szwarcbart, 2005).
Recording sites (blue dye marks) were localized by microscopic examination and then plotted on camera lucida drawings of serial sections for further analysis.
Boundaries of the TPC with adjacent cortical areas were charted onto camera lucida drawings of the sections at ×7.5 magnification.
In Figure 6 B schematic drawings of coronal sections of grafted animals indicate the location of 5-HT3A GFP+ grafted cells.
We then outlined the TLCd (as well as fiduciary marks used to align the drawings of consecutive sections) using a 20× (N.A. = 0.50) objective lens.
The extent of each injection relative to areal borders and white matter is shown in line drawings of selected section contours for each individual case in Supplementary Figure 6.
Drawings were made of sections showing electrode tracks related to the structure of the ACC and MT. A standard rat atlas (Paxinos and Watson 1998) was used as a reference for reconstruction of the stimulating and recording sites (Kung and Shyu 2002; Gao et al. 2006).
Drawings of double-labeled sections were made with an Axioscope microscope (Zeiss, Germany) equipped with a motorized X Y-sensitive stage and a video camera connected to a computerized image analysis system (Mercator; EX Y-sensitivea Rochelle, France).
Drawings of double-labeled sections were made with an Axioskop microscope (Zeiss, Germany) equipped with a motorized X Y-sensitive stage and a video camera connected to a computerized image analysis system (Mercator; EX Y-sensitivea Rochelle, France).
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