We need drastic regulation, technology to assist and have them both supplemented with massive changes in attitudes to how we store, sell and transport water.
But while he seeks to empower individual consumers, he stops short of calling for radical solutions that might level the playing field altogether: drastic regulation, or even nationalisation.
"Falls in income concentration due to economic downturns are temporary unless drastic regulation and tax policy changes are implemented," Mr. Saez said in his analysis.
In addition to biological consequences of RBP-J-mediated regulation, we also sought to investigate molecular mechanisms by which RBP-J regulated M2 gene expression especially expression of Arg1 given its role as a critical M2 effector molecule and its drastic regulation by RBP-J at the protein level (Fig. 4A).
Among the tested clusters (data not shown), only clusters 12 and 27 showed a drastic regulation.
Whereas cell-wall metabolism enzymes (the autolysin lytM, and several amidases lytA, sgtA and SA1759) showed drastic up-regulation, transpeptidase, acid techoic synthesis genes, the mur operon and several cap genes showed massive down-regulation after internalization (additional file 3).
The drastic up-regulation of YPMT1.34 and YPMT1.34A (more than 100-fold) is difficult to explain because no known functions have been attributed to these genes.
The reactivation of the LKB1-AMPK-mTOR pathway under low energy conditions by the inhibition of BRAF signaling led to a more pronounced effect that included a drastic down-regulation of Mcl-1 (Fig 6).
Accumulation of the degradation resistant β-catenin in the midbrain and rhombomere1 leads to drastic up-regulation of the expression of Wnt target genes, including Drapc1, Axin2 and Tcf1.
Interestingly, when the LKB1-AMPKα pathway was restored under low glucose conditions, the increased number of dead cells correlated with a stronger biochemical response including the complete de-phosphorylation of Bad, the stabilization of the non-phosphorylated BimEL isoform and the drastic down-regulation of Mcl-1 (Fig. 5D).
