This modified method is also used for the purification of a non-heme-binding apoMb mutant, demonstrating its efficiency when dealing with drastic mutations.
For drastic mutations, most of them are relevant to glycine or proline.
Thus, such drastic mutations are probably only of importance in the context of corrections for polyploidy that were discussed above and if mutation rates are already very high.
A continuous inbreeding experiment like the one we performed to reveal deleterious cryptic variation would be difficult with such drastic mutations, partly because homozygote offspring would be lethal.
Much of the genetic and phenotypic diversity evident in natural Arabidopsis accessions occurs as a result of drastic mutations, such as deletions and nucleotide changes resulting in the introduction of stop codons.
The early triumphs of human genetics and positional cloning involved rare, drastic, single mutations.
The seven drastic MxA mutations found in this study (L95P, P96S, G392V, V449G, P218S, R522C, and E632K) are likely to play important roles in tumorigenesis and development of corresponding human cancers, and therefore evolve to effective tumor-related biomarkers, although more biochemical and cellular assays are needed for their potential clinical applications.
The relatively small (3 °C) decreases in melting temperature by the quite drastic Asp56 deletion mutation and the R232A/P233G/L234R/D235P four-fold mutation may indicate that these residues are not in a so-called weak spot of the protein that is more sensitive for unfolding (Eijsink et al. 2004), although the higher B-factors had suggested it to be.
