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This cell line showed dramatically reduced expression of all three genes, expressed Nanog constitutively, and showed aberrant clonal morphology.
Most of the members of gap junction protein beta family exhibited dramatically reduced expression in EPT1 cells (Table 2).
Immunofluorescent analysis of 16HBE14o- cells, which usually express high levels of the epithelial tight junction proteins coxsackie and adenovirus receptor (CAR) and occludin and the adherens junction protein E-cadherin, showed dramatically reduced expression of these epithelial proteins after 72 h of treatment with TGF-β/EGF (Fig. 5A, B, D, E).
Curiously, a subset of these genes showed dramatically reduced expression in pollen; this set of genes includes those encoding AtCPSF160, AtCSTF77, and AtPABN3.
Confocal microscopy clearly indicated that the NUMB overexpressing HAT-7 cells had dramatically reduced expression of activated Notch 1 represented by the expression of green fluorescence).
Genes that had dramatically reduced expression values in M-ZE compared to the other ZE stages included TcLec1, TcLec2, TcWRI1, TcAGL15, and TcFUS3.
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Note that such cross-hybridizations will dramatically reduce expression intensities.
A priori, we predict that TE insertions into exons are likely to dramatically reduce expression levels.
Furthermore, co-expression of VHL dramatically reduced the expression levels of FLAG-tagged-p160 in 293T cells and this was abolished by a proteasome inhibitor, MG-132, suggesting that VHL induces proteasome-dependent degradation of p160 (Figure 2B).
We also found that stable re-expression of PEDF in the resistant cells re-sensitized them to the antiproliferative effects of tamoxifen and that re-expression of PEDF dramatically reduced the expression of the receptor tyrosine kinase RET along with p-AKT and pSer167ERα.
Results from this study showed that overexpression of NUMB dramatically reduced the expression of activated Notch 1 protein expression in HAT-7 cells.
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