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As shown in Figure 3B, Hsf1 targets with 'step' type HSEs (HSP12), or perfect HSEs (SSA3/4, HSP42, and HSP78), were increased dramatically for expression in these cells at 25°C compared to wild-type, whereas CUP1-1 (whash has 'gap' type HSEs) was nearly unaffected for expression under these conditions.
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We note that mRNA for two of the ATF4-sensitive genes shows a sexually dimorphic response to acarbose, with females responding more dramatically for ATF3 expression and less strongly than males with respect to ASNS.
This approach enables us to dramatically reduce the time and resources required for expression screening by eliminating the need to create recombinant baculovirus.
Among the plasmids, expression varied dramatically; for instance, we identified 34% and 54% of proteins encoded on lp54 and cp26, respectively, whereas proteins from the other 16 plasmids varied from 4% to 18%.
As shown in Figure 3A, by using two different siRNA sequences for each target, we dramatically decreased expression of NMMIIA, NMMIIB and NMMIIC without significant effects on expression of the other isoforms.
In this study, we found that Lhx1 deletion dramatically downregulates expression of several SCN-enriched neuropeptides crucial for circadian function.
For example, from Figure 4, removing the cross-hybridizing probes will dramatically change expression estimates in many different tissue types.
Although these genes accounted for a very low proportion of tobacco trichome ESTs, they all showed dramatically increased expression level compared to leaves.
Interestingly, we also observed that BPA treatment dramatically reduced expression of IGF-1, thereby reducing IGF/FSH synergy, thus providing additional support for BPA as an endocrine disruptor.
Reagent A results in a dramatically changed expression profile.
The truncated form exhibited dramatically enhanced expression and solubility.
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