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T cells primed in cervical and mediastinal lymph nodes were therefore allowed to leave the draining site, but not to re-enter in distal iliac lymph nodes, due to the blocking of L-selectin.
To this end, we measured cytokine production and surface marker expression of CD4+ T cells present in the Mandibular Lymphnodes (MLN), the draining site of the nasal mucosa where T cell immune deviation may be induced.
The anti-CD62L antibody treatment shows that primed T cells in cervical and mediastinal lymph nodes are allowed to leave the draining site, but not to re-enter in distal iliac lymph nodes, demonstrating that the dissemination of primed T cells in the iliac lymph nodes is mediated by the expression of CD62L.
Recent developments in SLN concept and technology have resulted in the application of this approach to define the first draining site or SLN to which a cancer may have metastasized [ 26, 27].
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The OVA-specific clonal expansion was assessed in draining and distal lymph nodes 5 days after nasal immunization, since we previously demonstrated the presence of proliferated T cells within non draining sites at this time point [15], [16].
Indeed, as shown in figure 2 C, the ratio between the amount of CD4+ or CD8+ TgN T cells measured in FTY720-treated versus untreated mice was >1 in draining sites and <1 in distal lymphoid organs, since the clonal expanded T cells could not disseminate due to the fingolimod effect.
Stimulation of the paracortical areas was not detected in the nodes draining sites of injection of a tumour not inducing concomitant immunity.
The drained site was instead colonized by a mature canopy of willow and birch.
Within the drained site, the transect was aligned perpendicular to the ditch network.
We observed the absence of Sphagnum moss cover at the drained site and a 77% reduction in total moss cover (Figure 2a).
The mature canopy of the drained site was comprised of seven different Salix (willow) species and two different types of Betula (birch).
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