To simulate drought stress, plants of DK151 were stressed by slowly draining the solution.
Then, 100 μl of serum blocking solution was added to each section, followed by incubation for 10 min and then draining the solution.
The bags were taken out of the solution and allowed to drain the excess solution for 4 – 6 h.
Drain the blocking solution from the slides and apply two drops of avidin solution (Avidin biotin blocking kit; Vector Laboratories; Dossenheim-Germany; SP2001) for 15 min.
Drain the biotin solution from slides.
Let it stand for no more than 5 minutes, then drain the bleach solution through the tap and into a bucket.
To check the ability of sustaining the preconcentration plug, the preconcentration was performed by draining the buffer solution for 120 s, and subsequently, the electric field was turned off.
Pre-packed resins were pre-washed by draining the packing solution (deionized water), flushing with 3 bed volumes of deionized water with a resistivity of >18 MΩ.cm followed by 0.1 M HCl, and flushing with at least 10 bed volumes of 4 M HCl.
Instead of experience and expertise being seen as an asset, it is implied that "costly" expert staff drain the resources of organisations, and solutions need to be found.
After draining this solution, the slides were incubated at room temperature with rabbit anti-myeloperoxidase (MPO, 1 : 100, Santa Cruz Biotechnologies, Santa Cruz, CA, USA), mouse anti- α smooth muscle actin (α-SMA) (1 : 1000, Sigma-Aldrich, St . Louis MO, USA), rabbit anti-TM (1 : 100, Santa Cruz), or rabbit anti-CD31 (1 : 200, Santa Cruz) overnight.
