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Multiple drafts were generated and revised until the test writers have reached consensus on the final draft.

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The L er-0 draft was generated by a combination of de novo assembly and reference-based mapping.

Draft assemblies were generated using Newbler 2.6.

Preliminary draft assemblies were generated with Velvet [ 80] (Table  1).

High coverage Illumina data were obtained for these four L. reuteri strains and draft assemblies were generated as described in Methods.

Known M. leprae polymorphisms were PCR amplified and Sanger sequenced, while draft genomes were generated by enriching for M. leprae DNA, and Illumina sequencing.

Draft genome sequences were generated from several BGstrains in our collection.

Furthermore, since many of the draft genome sequences were generated by whole genome shotgun assembly, we took care to identify redundant KRTAP sequences and excluded these from our analysis.

Sequence alignments against the P. taeda v1.01 draft genome assembly were generated to compare transcript to genome mapping of the P. patula v1.0 transcriptome assembly to that of other Pinus transcriptomes.

Draft gene models were generated with MAKER2 (Cantarel et al. 2008) using evidence from a SNAP (version 2010-07-28) hidden modelv model generated from the validated SN15 gene structures, a Genemark ES 2.3 hidden Markov model (Ter-Hovhannisyan et al. 2008) trained on each of the new genome sequences, and mRNA sequences from the set of validated SN15 genes.

Antibiotic resistance gene profiles were generated from each draft assembly, using ARDB Antibiotic Resistance Genes Database.

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