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So far, only four of the 31 communities worst affected by the disaster have completed draft reconstruction plans.
JAL had earlier submitted its own draft reconstruction plan, which included cuts of 6,800 jobs, or about 14percentt of its workers, but it failed to get a passing mark from Mr. Maehara.
The Blue Brain project, which recently published a first draft reconstruction and simulation of a portion of rat somatosensory cortex [1], has collected around 2000 biological reconstructions using standardized protocols.
Here we demonstrate that improved laboratory and bioinformatics protocols enable high-quality de novo draft reconstruction of genomes of uncultured taxa from complex microbial communities.
The first step was to build a draft reconstruction.
Second stage comprised of semi-automatically refinements of the draft reconstruction through gap analysis.
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The draft reconstructions were converted into condition-specific draft models [ 4].
The draft reconstructions were then manually curated and augmented with additional information taken from online databases and published literature.
Next we created draft reconstructions for each species using two different approaches as described in the result section.
The ratio of Recon 1 genes with a homologous gene as well as the ratio of reactions from Recon 1 included in the draft reconstructions for each mammal is shown in Figure 1b.
The sequence based genome annotation of F. tularensis subsp. holarctica (GenBank Accesion AM233362.1) was downloaded and served as the framework for the initial draft of reconstruction.
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