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Sequences were aligned using genome assemblers to produce draft assembly, followed by gap closing, quality improvement, and assembly verification.
It is not yet assembled into chromosomes, but a draft assembly of gene-rich regions has been constructed.
We used the PCAP-REP assembler [ 11] to obtain a draft assembly for whole-genome alignment.
Assembly accuracy of both the Matina 1-6 physical map and the Criollo draft assembly should also be verified.
After assembling the reads in successively larger regions, the result is a draft assembly with large contig sizes and relatively few misassemblies.
The final draft assembly contained 65 contigs in 65 scaffolds, totaling 9.064 Mbp in size.
Further analyses of our B. vibrans genome draft assembly revealed several sequences to encode putative sesquiterpene synthases (STS).
The initial draft assembly contained 25contigs in 25scaffolds.
Nineteen complete proviruses, designated RhERV-K, were found in the rhesus monkey (Macaca mulatta) draft assembly genome (Text S1).
An additional 528-primer walk reads were created as needed to address low quality regions of the draft assembly.
We believe therefore that the draft assembly of ACBU02 represents a near-complete genome for downstream analyses.
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