However, to date, there is no report on monocyte HLA-DR monitoring exclusively in neonates (< 28 days of life) who underwent cardiac surgery under cardiopulmonary bypass (CPB), which have a high risk of nosocomial infection.
So far, flow cytometry is considered the gold standard in HLA-DR monitoring.
The correlation between flow cytometry and PCR-based HLA-DR monitoring is stronger in the early phase of sepsis.
In this study, we evaluated an mRNA-based HLA-DR monitoring by quantitative real-time PCR (qRT-PCR) as an alternative method to traditional flow cytometry.
In patients with sepsis (n = 59), blood samples were collected simultaneously for HLA-DR monitoring by qRT-PCR (transcripts of HLA-DRA and CIITA) and flow cytometry.
These important limitations of traditional HLA-DR monitoring clearly hamper the clinical usage and feasability of larger multicenter studies required in this field.
An mRNA-based HLA-DR monitoring by polymerase chain reaction (PCR) would improve the clinical usage and facilitate conduction of large multicenter studies.
The different techniques of HLA-DR monitoring in this study correlate highly but are not analogous since we measure the HLA-DR expression at two different levels and in different blood cells.
In contrast, mHLA-DR monitors the surface expression only on monocytes.
DRs monitored community networks (e.g., local police, district administrators, village-level authorities, local media and partner NGOs) to identify incidents of intentional explosions as soon as they occurred.
