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We estimated that the amount of Zera-Xyl in transformed leaves collected at 7 dpi represents up to 9% of the total protein.
Based on trial infections, time points chosen for this study represent different stages of infection where 12 dpi represents early infection (pre-symptomatic), 32 dpi represents active systemic virus replication and movement (symptomatic) and 67 dpi represents a later infection stage (persistently symptomatic in T200 and recovery in TME3).
The sampling point 4 dpi represents the end of the acute phase of the disease, whereas the samples from 9 and 14 dpi represent the recovery phase (Fig. 1).
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The Sgt1-silenced plants showed an average late blight infection of 4.0 (± 0.0) after 7 dpi and 3.7 (± 0.7) after 10 dpi, representing ~70%and~73%3% infection, respectively.
We sampled two critical time-points of MD progression, 5 and 10 dpi, representing early cytolytic and latent stages of MD, respectively.
It is likely that the motor deficits observed at 1 and 3 dpi represent spinal shock from the surgery, while genotype differences in injury progression occur at 7 dpi and beyond.
The injured area at 60 dpi represented less than 5% of the ventricular area in WT hearts (2.5% ± 0.8%) but, in tert −/− hearts, was comparable with the injured area at 3 dpi (18% ± 2%; Figure 2C).
We assumed that the onset of necrosis in the tissue infiltrated with pTRA_e35STX at 6 dpi represented high levels of recombinant protein expression (like the stunted phenotype of transgenic plants) even though detected vIL-10 in the necrotic tissue was lower due to high levels of proteolytic activity and inefficient protein extraction.
DPIs represent a type of delivery system where the system's thinking approach integrating the device, the formulation and the patient represent a major challenge to be met.
The average score of the late blight infection on the Rar1-silenced plants was 7.3 (± 0.6) after 7 days post inoculation (dpi) and 6.9 (± 0.0) after 10 dpi, which represents ~13% and 18% foliage infection, respectively.
SA-β-gal positive cells (blue) appear at 7 days post-induction (dpi) and represent 80% of the total population by 12 dpi.
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