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Indeed, the mRNA expression patterns were skewed towards up-regulation in the infected groups, with 591 up-regulated genes and 246 down-regulated genes at 7 dpi (out of 36,991 genes analyzed); and 1,768 up-regulated genes and 744 down-regulated genes at 15 dpi (out of 35,577 genes analyzed).
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At 2 dpi, seven pigs out of ten had already fever which lasted until 28 dpi ranging from 40 to 42.1°C.
DPI buttons are out of the way but quickly accessed.
Antigen-specific proliferation was noted for the first time at 7 dpi in four out of six inoculated pigs.
The highest incidence was observed at 4 dpi with 4 out of 10 positive TGEV shedding piglets in each group.
At 14 dpi, five samples out of seven were positive with viral titres between 102.3 TCID50/100 mg and 103.6 TCID50/100 mg.
At 28 dpi only one pig out of six was still viremic with low viral titre (101.6 TCID50/ml).
Viral titres in sera of PRRSV (Lena -inoculated animaLena -inoculatedtected animalsi in nine pigs out of 10.
At 21 dpi, three tonsillar scrapings out of five were PRRSV positive with viral titres ranging from 102.5 TCID50/100 mg to 103.1 TCID50/100 mg.
In the ciprofloxacin DPI group, 14 (35%) out of 40 subjects reported pathogen eradication at end of treatment versus four (8%) out of 49 in the placebo group (p=0.001).
Mean colony counts of selected pathogens at baseline and at EOT are presented in figure 3. Fewer subjects reported at least one exacerbation throughout the study in the ciprofloxacin DPI group (22 (36.7%) out of 60 subjects) than in the placebo group (25 (39.1%) out of 64 subjects; hazard ratio estimate 0.802, 95% CI 0.443 1.454; p=0.443 1.454
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