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Flowers from fields grown (2005) cotton plants (DES119 and ST4793R) were tagged with the date of anthesis and harvested 0 dpa, 1 dpa or 10 dpa.
Blastemas (including about 200 μm of the distal stump tissue) of 20 tadpoles each were recovered at 0 dpa, 1 dpa, 3 dpa and 5 dpa and pooled for RNA extraction.
Ovules harvested from bolls -1 dpa, 0 dpa, 1 dpa or 3 dpa were washed briefly in phosphate buffered saline (PBS) and incubated for 30 min in 25 μM DiOC or in water.
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Cotton bolls were collected at fibre initiation (−2, -1, 0, 1 and 2 dpa), elongation (5, 10 and 15 dpa) and secondary cell wall synthesis (20 dpa) stages.
MCU5 at fibre initiation (0 dpa), elongation (5, 10 and 15 dpa) and SCW synthesis stage (20 dpa) was carried out using Affymetrix cotton GeneChip genome array.
In both species, fiber development occurs in four overlapping stages; initiation (-3 to 5 dpa), elongation (3 to 21 dpa), secondary cell wall synthesis (14 to 45 dpa), and maturation (40 to 55 dpa) [ 4].
Unpollinated pea pistil senescence involves programmed cell death, which initiates at 2-3 days post-anthesis (DPA) [ 1, 5, 6].
Similarly, the average degree of polymerization (DP) increased from 3 DPA (12.3) to 12 DPA (15.0), and then did not differ significantly up to 30 DPA (15.0).
Recommended uni-directional (cardioid or supercardioid patterns) microphones: Sony ECM66BC, Audio Technica AT898C and AT831C, Sure WL184, DPA 4080 and DPA 4022.
Irradiation experiments were performed to 0.1 dpa at 350 °C, with a damage rate of 4.57 × 10−6 dpa s−1.
In Pyramimonas sp. also small amounts (3%) of docosapentaenoic acid (DPA; 22 5 n-3)) were found.
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