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"I fear right now, human nature being what it is, that downstream we could find ourselves in the same situation".
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25, 35 If PIK3CA is functionally downstream of TP53, we could conceivably treat patients with TP53-mutated cancers with PI3k pathway inhibitors and achieve a therapeutic efficacy.
For example, in our previous studies, we found many genes that showed sex differences in transcript abundance downstream of tra, but we could not place them downstream of dsx or fru.
While the binding sites for these regulators reside in promoter and enhancer regions up- or downstream of the gene, we could identify a regulatory element within an intronic region of ISL1.
One evening we would sail to have the best sunset, or find the best spot to fish for piranhas and by morning we would be further downstream at a reserve where we could canoe in to a hidden spot for breakfast where a table of local fruits, pastries, and coffee would already be laid out for us.
By using inhibitors for the PDGFR itself and for known targets downstream of the PDGF receptor, we could verify that miR-21 was indeed decreased.
After nested amplification with gene-specific primers located downstream of the second AUG, we could amplify in HeLa cells a specific product of about 250 bp, which was absent from the minus TAP control reaction.
Since the T allele was only detected when the flanking primer was located not more than 1 kb upstream or downstream of the polymorphic nucleotide, we could estimate the size of the transposed fragment to approximately 2 kb and it does not overlap the Grey duplication.
However, we could demonstrate that the downstream process autolysis has an influence on E. coli binding (P < 0.001).
We could identify DMRs upstream of PDE120 and one DMR downstream of ATCDC48 that could potentially act as ICRs.
And we like simple mechanisms because we think of them as implementable what downstream neurons could do.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com