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Colonies were grown in ES medium and those exhibiting relatively homogeneous epithelium morphology were considered as promising epithelial-like cells [16] and picked for further expansion and downstream study.
As a final quality control for the purified RNA samples, only cDNA falling within ± 1.5 CT of the median value for 18S for all samples were used in the downstream study.
Similar(58)
RSCU values of all amino acids in 10 species' CDS sequence were calculated separately for downstream studies as shown in Fig. 4.
The methods described here will enable downstream studies that require consistent and defined neural cell populations.
In summary, CoESPRIT is an efficient method for identification of purifiable soluble complexes at yields compatible with downstream studies.
How the presence of lamin A influences the expression of an actin-bundling protein is the subject of downstream studies.
Scale up of partially soluble ESPRIT hits to larger culture volumes [38] or lysis buffer and strain optimisation [46] can overcome these problems to yield sufficient quantities for downstream studies.
The ORF clones are a resource for downstream studies.
Mammalian cell culture material is often difficult to produce accurately and reproducibly for downstream studies.
Wild-type tobacco plants (Nicotiana tabacum cv. Samsun) were used for the collection of tissue samples for all downstream studies.
This added benefit therefore allowed histologic correlation of tissue with any downstream studies performed on the exfoliated cells.
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