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P dnaK prolonged to the downstream sequence of initiation coden and carried additional optimal RBS and AS of R4a.
Then P dnaK prolonged to the downstream sequence of initiation coden and carried additional optimal RBS and AS of R4a (P dnaK -2SD) was tested for its strength.
Sediment accumulation at confluence zones is not determined by the catchment area of tributaries or by landslide area; rather, it is determined by valley confinement of the trunk stream at the confluence and the associated downstream sequence of river types.
One design, in particular, that had YscF fused to the downstream sequence of F1, produced better protection than separate F1 or YscF DNA vaccines, suggesting a potential synergistic effect between these two antigens.
A long-distance inverse polymerase chain reaction (PCR) using nested primer combinations designed for each constant gene of IGH showed that Cγ4 was juxtaposed to the downstream sequence of the BCL2A1 (BCL2-related protein A1) gene through the Sγ4 switch region.
The ribozyme was designed to trans-splice its 3′ tagging sequence comprising a new coding RNA, such as firefly luciferase transcript, that is linked to the 3′ part of the HCV 5′ UTR encompassing the downstream sequence of the targeted residue in the IRES.
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The upstream and downstream sequences of the nprE gene were inserted into plasmid pUK, yielding nprE knockout vector pUNprEK.
A 6,334-bp 6,334-bpDNA fragenomicontaining the entire GhDNA coding region and upstream and downstream sequences ofragmentwas subcontainingm the BAC clonentire7658 and inserted into the binary vector pCAMBIA1300 to Ghd10ate the transformation vecodingGhd10 foregionin the complementandon test.
Furthermore, BLAT search using Lf cosmids sequences retrieved up- and downstream sequences of the PmHhb genes.
The siRNA for DNA-PKcs (5'-GAU CGC ACC UUA CUC UGU U-3') targets the sequences of 352 base and downstream sequences of human DNA-PKcs mRNA [87].
Clones were selected one day later, and sequenced using the forward and reverse primers from upstream and downstream sequences of the antisense insertion.
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