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The Pat1 promoter has been described previously [32] [29], while the Pat2 promoter is a 648-bp fragment from the P24 promoter [33] flanked by Sal I (upstream) and Bam HI (downstream) restriction sites.
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This analysis indicated that in maize several enzymes, namely HincII, RsaI, and AluI show considerable reductions in guanine one to two bp upstream and cytosine one to two bp downstream of restriction motifs.
Differences between predicted and covered datasets in guanine ratios 1-2 bases upstream and cytosine ratios 1-2 bases downstream of restriction motifs were potentially due to methylation, as plant methylation can occur at CpG and CpNpG motifs.
We synthesized two sequences corresponding to BABAM1 exon 2, 50 base pairs of flanking 3′ intronic sequence and 100 base pairs of flanking 5′ intronic sequence, one with the major (C) and one with the minor (T) allele of rs10424178, with an upstream SalI and a downstream XbaI restriction enzyme site (purchased from IDT DNA).
Drop break-up downstream of the restriction has been studied using a high-speed trajectography.
It was observed that the mean drop diameter downstream of the restriction linearly increases as a function of the inverse of the square root of the pressure drop.
As fluid converges toward the opening of an orifice, it builds up considerable inward radial momentum that causes the flow stream to continue to flow "inwards" for a distance downstream of the restriction (Darby 2001; Sahin and Ceyhan 1996).
A RAD locus is a region downstream of a restriction site.
Ppar-mediated signaling is a well documented molecular event downstream of calorie restriction [ 56, 57].
Specific primers for TaSK2 sequences were designed upstream and downstream of this restriction site (Table 2).
This fragment was inserted into a pcDNA3 vector coding for the SV5 protein tag [ 20, 21], downstream SV5 between restriction sites BamHI/ EcoRI.
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