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Groundwater samples were collected on a monthly basis over a two year period for chemical and microbiological analysis from nested multi-horizon sampling boreholes upstream and downstream of the study sites.
The turbidity maximum zone was located downstream of the study site since thick slackwater mud drapes that characterize the seaward part of the fluvial to tidal transition zone are not present; only a few thin mud drapes are found at the study locality.
Group D consists of 6 samples (sample no. 21, 22, 20, 24, 14, and 19) all are from downstream of the study area; they were highly polluted due to industrial activities, municipal sewage, and runoff from irrigation lands.
In our study, incorporating immigration from downstream of the study area was observed to rescue the tributary/mainstem population from extinction resulting from fragmentation under most scenarios.
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Three intermediate transects (B D; Figure 1) were established downstream of A. The study reach included one perennial (west bank) and one transient (east bank) tributary.
ODP shows an opposite trend and is at a maximum (0.7) in the downstream part of the study area.
A mountainous and hilly dissected terrain with steep slopes characterises the upper stream whereas an undulating topography and gentle slopes form the downstream parts of the study watershed.
The downstream end of the study area is bounded by a small waterfall (1-m tall, passable by fish), and the upstream end is unbounded for several km.
Dissimilarities between the studies include the use of 1000 bp upstream and 200 bp downstream of the TSS (this study) versus 1500 bp upstream and 1500 bp downstream by Saxonov et al. We calculated normalised CpG count for the same window, but this did not change the relative distribution of promoters.
In order to investigate processes occuring downstream of the CSSP, we studied the transcriptional responses towards sMyc-, nsMyc-, and s/nsMyc-LCOs in nsp1-1 and ram1-1 plantlet roots.
As we show, PHH grown in 3D microfluidic cultures do express and maintain stable expression levels of innate immune receptors and downstream effectors, enabling the study of host/pathogen interactions.
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