Exact(2)
The oligonucleotide primers were as follows: (1) upstream for the first and second PCR, 5′-TAAACTTGTGGTAGTTGGAACT-3′; (2) downstream for the first PCR, 5′-GTTGGATCATATTCGTACAC-3′ and (3) downstream for the second PCR, 5′-CAAATGATCTGAATTAGCTG-3′.
In both cases, the local regulation causes an increase of the metabolic flux through the pathway upstream (for the first class) or downstream (for the second class) the metabolite effector (see proposition 2 and 4 Additional file 7).
Similar(58)
b,c, The apparent symmetry for regions up- and downstream of the first exons shown in a disappears when only regions annotated as promoters and introns (upstream and downstream, respectively, of the exons) are considered in particular, in the immediate vicinity of the coding region (c).
For each of the murine untranslated exons, the region spanning 500 bp upstream and 100 bp downstream of the first nucleotide were analysed for TF-binding sites and the transcription start site (TSS) prediction score within this region was assessed (Figure 7B).
In a Jan . 11interim update the company noted that downstream results for the fourth quarter would be sharply lower, owing to a drastic fall in refining revenues.
The first PCR was as follows: Taking Chinese Spring DNA as the template, AP Primer as the upstream primer, and TaGW2-SP1 as downstream primer for the first PCR.
Thermocycling conditions were 94°C for 5 min; 40 cycles of 94°C for 30 sec, 51°C for 30 sec, 72°C for 30 sec; 72°C for 5 min. PC03/PC04 primers for β-Globin, JA9AE, JA9B are the upstream primers and JA12A, JA12B are the downstream primer for the first round PCR.
The resulting increases in "fluvial flow" downstream created the second type of reason for closing the barrier – protecting vulnerable locations just upstream from Teddington, such as the village of Thames Ditton and the residential Trowlock Island.
Consolidation of the second-generation with the first-generation process can potentially reduce the downstream processing cost for the second-generation process as well as providing the first-generation process with energy.
The maximum distance between two samples of an identified individual was 0.83 that is considerably greater than the position of the first plateau but slightly less than that for the second plateau downstream.
To test for possible downstream regulation, the first non-translated exon of Gpat2 mRNA as well as the first intron were included in the promoter constructs [ 16].
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