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The IRES localizes to a downstream portion of 5' untranslated region (UTR) and a part of the early downstream coding region of the transcript.
Analysis of these introns confirmed the prior observation of longer first introns and also revealed that while the first intron of the coding region remains significantly longer than downstream coding introns, it is the first intron of the 5' UTR, if present, that is the longest intron within a transcript (Figure 3).
In this case, the first two exons, containing the start codon and part of the downstream coding region, were skipped.
This exon might be completely novel or, more probably, has been insulated from the downstream coding region by acquisition of an intron.
Whether these uORFs or other 5' leader motifs are responsible for the silencing of the downstream coding region remains an important question.
MeCP2 is also required for expression of EZH2 and H3K27me3 modifications in the downstream coding region of the gene that impede transcriptional elongation.
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The rat CB1 open reading frame, fused downstream the coding region of the hemagglutinin (HA -tag, replaced GFP in pAAV-Stop-GFP to obtain pAAV-CBA-Stop-CB1-WPRE-bGHA -tagAV-Stop-CB1).
REPs are predominantly located downstream from coding regions, and many are plausibly transcribed and function as RNA elements.
The incorporation of the polyadenylation signal prevents the incorporation of downstream exons coding for multiple zinc fingers (Fig. 1).
We have developed a novel tool that searches for cis-element candidates in the upstream, downstream, or coding regions of differentially regulated genes.
In both genomes, spf is flanked by polA as the nearest upstream neighbor and yihA (or the engB homolog) as the nearest downstream protein coding gene.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com