The phrase "downstream assays" is correct and usable in written English.
It is typically used in scientific and research contexts to refer to tests or analyses that occur later in a process, often following an initial experiment or procedure.
Example: "After conducting the initial screening, we proceeded to perform downstream assays to evaluate the efficacy of the compounds."
Alternatives: "subsequent tests" or "follow-up assays."
To prepare for downstream assays, organoids are then released from Matrigel by incubating at 37 °C for 40 min in 50 µl of 5 mg/mL dispase (Life Technologies 17105-0411).
In-flow counting enables a rapid assessment of the cancer-associated large circulating cells in a sample within minutes to determine whether standard downstream assays such as cytological and cytogenetic analyses that are more time consuming and costly are warranted.
The ideal for characterizing therapeutic sensitivity would allow for: realtime decision making, identification of rare subpopulations with therapeutic resistance, analysis of very small samples (e.g. MRD), and maintains viability individual cells for downstream assays to characterize phenotypic, genotypic, transcriptional and other determinants of sensitivity.
The electrophoretic sample separation of nucleic acids will continue to be an important part of the sample-to-answer workflow, providing cleaner analytes for downstream assays like clinical diagnostics, polymerase chain reaction (PCR) and sequencing.
Once converted, downstream assays could be applied, including not only restriction enzyme digestion but also currently-available sequencing-based approaches.
PMNs were suspended in desired medium for downstream assays, and the purity/viability was typically >90% as evaluated by flow cytometry.
Remaining challenges are the barcoding of live, unprocessed cells to increase downstream assay performance combined with the flexibility of the approach towards a broad range of cell types.
Therefore, the downstream assay performance was not compromised.
Forty-eight hours after transfection, cells were harvested and prepared for the appropriate downstream assay.
Forty-eight hours after transfection, the cells were prepared for the appropriate downstream assay.
This is a critical insight that must be accommodated when the downstream assay is based on PCR enrichment, as it is for many targeted NGS assays.
