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Cells were analysed 1 3 days after transfection, depending on the downstream application.
Moreover, accessing the data needed for a specific downstream application scenario is a challenging task in large-scale BIM projects.
Fractions were diluted with 20 ml of PBS and centrifuged at 100,000 × g for 1.5 h at 4 °C and resuspended either in 50 μl of PBS or 20 μl of RIPA buffer depending on downstream application.
Hence, for a downstream application to handle various types of input, multiple measures of semantic similarity are needed, measures that often use different internal representations or have different output scales.
The paper addresses the issue of numerical and statistical modelling of extreme tropical cyclone winds in coastal regions, leading to the estimation of design return periods of gust wind speeds and the downstream application of risk models.
Reverse transcriptase polymerase chain reaction (RT-PCR) is the most common molecular downstream application assay used to assess both quality and quantity of mRNA transcript, which heavily relies on using a sequence of an endogenous gene as a reference.
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Mosquitoes were collected at 2, 6 and 12 dpi for downstream applications.
However, depending on the downstream applications, certain technologies have advantages over others.
The H2/CO molar ratio could be adjusted by the CO2/H2O feed ratio for downstream applications.
Such a definition can also be used for several downstream applications.
For almost all downstream applications a substantial reduction of the tar concentration is required.
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