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Some have downloaded Signal as part of organizing efforts for a wave of protests against Donald Trump's election in recent days.
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The data for AfCS double ligand screen of phosphoproteins was downloaded from Signaling Gateway website at [ 16] [see Additional file 1].
To correct for lane-to-lane variation in total protein loaded, in the downloaded dataset all signal intensities were already normalized against the signal for Rho-GDI detected by a phosphorylation insensitive antibody.
Figure 3C shows profiles based on ENCODE X-ChIP-seq data as processed by the ENCODE uniform processing pipelines and downloaded as 'raw signal', our high-resolution X-ChIP-seq stacked read data, and raw ChIP-exo data around a representative CTCF motif.
For that purpose, we collected 24 signaling pathways from Reactome [ 32] (downloaded at 19/05/2014), 35 signaling pathways from KEGG [ 33, 34] (downloaded at 21/10/2014), and 63 signaling pathways from WikiPathway [ 35] (downloaded at 16/10/2014) databases.
These data were downloaded as uniformly processed signal files with normalized scores of nucleosome-occupancy (MNase-seq) or depletion (FAIRE-seq) for each base.
CDX2 ChIP-seq data from CACO2 cells (Gene Expression Omnibus series GSE23436; Verzi et al., 2010) and TCF7L2 data from LS147T cells (series GSE18481; Mokry et al., 2010) were downloaded and mined for signals in the GREM1 gene and surrounding region.
This system allows me to not only program sequences of movements/functions for display and playback, it can also be controlled live through iPhone, iPad or other mobile devices (with a downloaded app) via dedicated wifi signal.
All input-normalized ChIP-seq signal tracks were downloaded from the ENCODE-X interactive faceted browser: http://encode-x.med.harvard.edu/data_sets/chromatin/. The original fly and worm ChIP-seq data were in 10-bp resolution.
Signaling Pathway Impact Analysis (SPIA) algorithm [ 49, 50] was applied to find the specific KEGG signaling pathways (database downloaded from KEGG's website on: 03/21/2012) that included the differential expressed genes related to cell proliferation.
The audio signal data [30] is downloaded from the UCI database.
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