Nuclear applications of boron carbide include shielding, control rods and shut-down pellets.
A cross between a meat grinder and a pasta maker, the device can crunch any number of things down into pellets – from plastic toy blocks to unwanted 3D printed objects – and extrude the pellets into filament.
I am not sure why — maybe it was those peanuts going down like pellets of lead.
For IHC of cell lines, 30 million cells per line were fixed in 4% paraformaldehyde for 10 minutes, washed with 70% ethanol, and spun down into pellets.
Thirty million cells were spun down and pellets were washed once with cold PBS before being frozen in dry ice.
Formalin-fixed cells spun down into pellets and paraffin-embedded, FFPE tissue samples or TMAs sectioned on glass slides were deparaffinised and rehydrated.
After 48 72 hr, cells were harvested by scraping in cold PBS, spun down, and pellets were then snap frozen in liquid nitrogen and stored at −80°C.
Cells were spanned down and pellets resuspended gently in 450 μl of sterile ddH2O, 50 μl of 3.5 N NaOH and incubated for 5 min at room temperature.
The isotopically labeled cultures were allowed to equilibrate for 24 h and then spun down into pellets and the supernatant discarded.
After quenching with 0.125 M glycine, the cell pellet are resuspended in lysis buffer (50 mM Hepes, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% TX-100, and proteinase inhibitors) for 10 min. After spin down the pellets are further resuspended in 10 mM Tris, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA.
