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Firstly, make-before-break approach is used that reduces the latency of link down detection after cluster change.
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Most keyboards use a simple method of key-down detection that results in no more than two being pressable at a time.
Rac1 activity was determined using an Active Rac1 Pull-Down Detection Kit (Thermo Scientific, Pierce, Rockford, IL, USA) as per the manufacturer's protocol.
According to Fig. 5, the (200) and (311) peaks of the retained austenite in the CHT sample can be observed, but this phase completely transformed to martensite and its percentage fell down the detection limit of the XRD techniques (<1 %).
Given the sensitivity restrictions of the antibody pull down and detection methodologies used, it is possible that some less abundant proteins were not identified.
Rac1 activity was measured using a Active Rac1 Pull-Down and Detection Kit (Pierce).
Samples were treated and analyzed with the Active Rho Pull-Down and Detection kit (Pierce Biotechnology, Rockford, IL, USA).
Rac1-GTP assays were performed with the Active Rac1 Pull-Down and Detection Kit using the manufacturer's recommendations (Thermo Scientific).
Affinity-based enrichment by pull-down and detection by western blotting are detailed in Extended Experimental Procedures.
Cdc42 activation was determined using an Active Cdc42 Pull-Down and Detection Kit (Thermoscientific) as per manufacturer's instructions.
Active Cdc42 levels were determined using the Active Cdc42 Pull-Down and Detection Kit (Pierce-Thermo Fisher Scientific, Waltham, MA, USA).
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