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One subpopulation displayed stem-like features with a slower cell doubling rate, increased sphere formation capability, and resistance to G2-M arrest induced by a mitosis inhibitor.
Chambers, SAM; Shaw, BR, "Levels of histone H4 diacetylation decrease dramatically during sea urchin embryonic development and correlate with cell doubling rate", The Journal of Biological Chemistry, 1984, 259(213458-134633463 [abs].
Plasmid stability or maintenance is primarily dependent on its segregational stability in which the plasmid must propagate in pace with the doubling rate of their host cells to be stably maintained in a cell population.
We found that the addition of IL-18 to culture medium did not affect the population doubling rate of Tregs in vitro after anti-CD3/CD28 stimulation, in the presence or absence of IL-2 (data not shown).
The doubling rate of Clone #82 (2 divisions/week) was only half as great as that of the parental cells (4 PD/week) (Fig. 1B) showing that the insertion also had adverse phenotypic effects that significantly slowed cell growth.
The stability test was conducted under optimal growth conditions for the labeled strains which generated a much higher cell doubling rate than in most environmental situations in which the labeled strain is intended to be used.
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Weekend doubles rates start at $259 on Fridays and $489 on Saturdays.
The double rate for its four rooms with bath is $21 a person.
The standard double rate, $79 weekdays and $69 weekends, includes breakfast.
Two adults and two children or three adults can stay for the standard double rate.
The double rate averages $200.
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