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If the stock tanks, investor has to keep buying it, even doubling number of shares he had agreed to buy each day.
The doubling number was calculated as follows: doubling number = log2 (Nt×DF)/N0, where Nt was the cell density at each time point, N0 was the cell density at t = 0 (i.e. 2×105), and DF was the dilution factor (calculated as the cell density before dilution divided by the cell density after dilution).
BJ WT cells at population doubling number 5 were transfected with 60 nM miR-143 mimic or negative control miRNA mimic (Dharmacon) for 6 hours in Opti-MEMI in the presence of 3 µg/ml Lipofectamine 2000.
Moreover, these five cell lines are now growing with a population doubling number greater than 200.
OD at each time point also was measured for each strain to estimate cell doubling number.
To determine the population doubling number, 4 × 10 cells were plated in 3 cm plates.
Similar(46)
The calculation results were tested with a doubled number of pieces.
Every doubled number should be divisible by two.
Start by doubling the number of teams in the tournament.
"Doubling the number of first-time buyers in our country.
Ten hotels have opened downtown, doubling the number of rooms.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com