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The population doubling level during each passage was calculated according to the formula log (final cell number/3×105)/log2.
The increase in population doubling level (ΔPDL), was calculated according to the formula ΔPDL = log(nf/n0)/log2, where n0 is the initial number of cells and nf is the final number of cells.
Additional information about the degree of differentiation comes from the estimated population doubling level and passage number, which act as a proxy for cell age.
For the Illumina® HumanMethylation27 BeadChip, three replicates per population doubling level were studied and significant difference in site-specific methylation levels were determined as described above.
Cells were defined to be immortalised when the population doubling level of the transduced cells surpassed 100 population doubling levels (Kyo et al, 2003).
No substantial species differences in passage number or estimated population doubling level distinguish the cells used in this study (fig. 4 C), suggesting that these factors are unlikely to account for the phenotypic difference between species.
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Cells were used in passages 2 12, corresponding to cumulative population doubling levels of 3 27 [ 32].
The assay was performed five times representing a broad range of population doubling levels (PDL 72, 93, 95 104 and 135), each performed with two replicates.
Doubling the level of economic energy, per school.
He is more than doubling the level of eligibility," Takano told HuffPost.
"So I think the doubles level has dropped, actually.
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