Exact(4)
Colchicine, as a chromosome doubling agent, has been widely applied to induce plant polyploidy because it disturbs the mitosis at the anaphase stage to result in chromosome doubling (Nigel et al. 2007; Pour et al. 2012).
He produced an artificial autotetraploid grass from the diploid species Ehrharta erecta through treatment with the chromosome doubling agent colchicine.
The near-allohexaploid hybrid was subjected to microspore culture according to protocols of Takahira et al. [ 36] and Cousin and Nelson [ 26] (using the chromosome doubling agent, colchicine), and resulted in a population of 75 microspore-derived (MD) progeny (Generation 4).
The most common doubling agent is colchicine which is traditionally applied in vivo[ 10].
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