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The deletion of comI had a beneficial effect on the transformability, as CM1 displayed a doubled transformation frequency of 201% ± 4.6, an effect that is nevertheless 50-fold lower than the effect observed in B. subtilis NCIB3610, in which the deletion of comI resulted in an approximately 100-fold increase of the strain's transformability (Konkol et al. 2013).
A comparable result for the same cultivar of barley, doubled transformation efficiency with Agrobacterium compared to particle bombardment, was obtained by Travella et al. [ 22].
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Since the initial plasmid preparations contained not only the pUT18 prey plasmids, but the pKT25_ dynA bait plasmid as well, the empty vector control usually yielded a mixture of blue and white colonies due to double transformation events.
As the deletion of comI in B. licheniformis DSM13 doubled the transformation efficiency rather than increasing the efficiency 100-fold, as for ComI3610 (Konkol et al. 2013), the intracellular level of ComIDSM13 might be more strictly controlled.
But, in this late summer, there has been a double transformation.
Europe generally requires what is known as a "double transformation" in goods for them to be considered made in a certain region.
The cotransfer ratio of a selected marker with an unselected marker on the opposite side of the genetic map of the B. subtilis chromosome was about 5.6% and could be interpreted as congression (double transformation) ratio.
The p n p double transformation and ultrasensitive insensitive gas sensing of reduced-graphene-oxide-flake stacked films (rGOFSFs) were controlled by changing the annealing temperature from 100 to 400 °C on a sodium silicate substrate, as a result of the competition between the thermal reduction, decomposition, and diffusion of Na, as determined from comprehensive microstructure characterization.
China is experiencing a double transformation in demography and family patterns.
A double transformation of empty vectors served as negative control.
Positive control was a double transformation of pUT18C_zip and pKT25_zip (Euromedex), encoding an interacting leucine zipper.
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