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Furthermore, FACS experiments on intact vesicles doubled labelled with A-SMase and annexin V revealed that A-SMase immunoreactivity represented 87±5% of total MPs exposing PS (not shown), indicating that the presence of the enzyme on MPs is instrumental for vesicle budding from PM.
Similarly, we could observe in doubled labelled immunofluorescence images, that cells that expressed sLex were negative for E-cadherin and vice-versa.
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No significant differences were found in the number of NeuN/BrdU double-labeled neurons.
Chondrocyte apoptosis was identified by FITC-annexin V/PI double-labeled assay.
The apoptosis rate was determined using annexin V-FITC/PI double-labeled flow cytometry (FCM).
Cryostat sections were double labelled with DAB and cell-specific antibodies to quantify ROS-producing cells.
Double labelled immunofluorescence staining was performed to analyse the combined expression of sLex and E-Cadherin.
Nuclei that were double labelled with TUNEL and DAPI were considered to be TUNEL positive.
For double labelled sections, images were collected using sequential acquisition between stacks.
T cells from each donor were double labeled for tetramer and IC-IFNγ (Figure 9).
Similar results were also obtained when synaptic vesicles were double labeled with APP and synaptophysin.
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