Sentence examples for double transfection protocol from inspiring English sources

The siRNA double transfection protocol with cells harvested on day 5 was as described by Motley et al. [ 17] and used previously [ 14], except that medium containing 20% FCS was added immediately following transfection, not after 4 h.

The double transfection (IRF5 siRNA2) protocol, harvested at 48 h post-transfection, was used in all further experiments since it gave approximately 80%reductionn.

PANC-1 cells were transfected using a reverse transfection protocol.

Cells were transfected with 10 µg of vector, according to a conventional CaCl2 transfection protocol.

The manufacturer's transfection protocol was followed.

The siRNA transfection protocol was followed with slight modifications [60].

For siRNA transfection, a 'double hit' oligonucleotide transfection protocol, using Oligofectamine transfection reagent (Invitrogen), was employed as previously described [ 19].

A 'double hit' oligonucleotide transfection protocol using oligofectamine transfection reagent (Invitrogen) was employed for siRNA transfection, as described previously (12), except prior to luciferase assays, where a 'single hit' protocol was used.

A single knockdown transfection protocol was used.

This double transfection procedure resulted in a transfection efficiency of approximately 60 70%.

Transfection was performed using Dharmafect 1 (Dharmacon, T-2001-01) in a reverse transfection protocol.