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Nucleotide-free MCAK was used at a concentration more than double the KM to ensure substrate binding was not limiting.
Since the concentration of xylose during our experiments was on average double the Km the transporters would be operating at 2/3 of the maximum velocity, which corresponds to approximately 0.26 g/g CDW/h (assuming a single transporter with Vmax = 110 nmol/min/mg protein [ 42] and a cellular protein content of 40%).
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In contrast to these signatures, the overall scale of the current sheet of event (1) was relatively large, more than a factor of 10 larger than the ion-gyro scale (i.e., ~70 km), and at least double the ion inertia length, (i.e., ~600 km).
The proposed 'Euro 6' tests would allow diesel cars to emit more than double the bloc's '80 mg per km' standard for NOx emissions from 2019, and more than 50% above it indefinitely from 2021.
One plan is to roughly double the LHC energy by ripping out the current magnets in the current 27 km tunnel and putting in more powerful magnets.
Grain samples, collected from fields in the Kiev region, approximately 50 km (31 miles) from Chernobyl, had radioactive isotope levels that were, in some cases, more than double the limit for human consumption.
"Doubling the distance to the grid from 50 km to 100 km typically increases [negative] impacts per kWh by 3-53-5%it concluded.
Between 1950 and 1990 China doubled the route-length of its national system to some 54,000 km (33,500 miles), and between 1990 and 2010 it doubled its route-length again, to approximately 110,000 km (66,000 miles).
Using the data mentioned above and applying double-reciprocal Lineweaver-Burk plots, the Km and Vmax values of rMsEno were determined to be 1.1 × 10−3 M and 0.739 μmol/L/min, respectively.
Thus, the Km values for hSMUG1 on double-stranded DNA containing U : G mismatches is not lower than the Km for hUNG2 tested under different conditions, including different concentrations of Mg2+.
If the hydrogen bond of the substrate carboxylate to S416 or S419 is also abolished in double mutants, Km further increases to about 1000-fold over the wild-type level (Fig. 4a).
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