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The AP2 family contains proteins with a double, tandem repeated, AP2 domain [ 5].
Reliable discrimination of point mutations has been achieved by double tandem hybridization with a set of seven-mer probes [ 23, 24].
In the current study the double tandem hybridization assay was applied to genomic DNA samples extracted from a variety of sources, including blood cells, cell lines and plasmids.
Thus, with exon 7 a single tandem hybridization was conducted with the glass-tethered 7-mer capture probes, whereas with exon 8 double tandem hybridization was conducted.
The comparison for double tandem repeats was done for motif lengths where the minimum locus contained two repeats, i.e., where the motif was at least eight nucleotides long.
We have developed an oligonucleotide microarray (genosensor) utilizing a double tandem hybridization technique to search for 9 point mutations located in the most frequently altered codons of the TP53 gene.
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