Sentence examples for double tagging from inspiring English sources

Introduction of a double tagging and dual affinity chromatographic procedure has permitted the engineering and isolation of heterodimeric fatty acid synthases carrying different mutations on each subunit.

These included failure to correctly attach tags (Farrington et al. 1993), double tagging (Handford 1994; Huber 2006), or attaching tags so that they cannot be easily removed (Bamfield 1992; Beck 2006; Beck and Palmer 2010).

To achieve double tagging the following genetic manipulations were performed in the CB010 background (Matα pep4:: HISG prb1:: LEU2 prcan1 HISG can1 ade2 trp1 ura3 his3 leu2 112eu2–112).

The introduction of double tags only marginally altered the affinity of the enzymes for their substrates, and the double-tagged proteins were enzymatically active in both soluble and immobilized forms.

"It was kind of a weird play," Napoli said of the double tag at third.

Protein purification was achieved using a nickel affinity column and, for the first time, direct quantification of ChABC down to 0.1 nM was attained using an in-house HIS/FLAG double tag ELISA.

h, Schematic of use of amber-suppressor tRNAs to introduce unnatural amino acids that can be clicked with fluorophores (h, top; see Methods), and schematic comparison between the Q3 and A1 double tag used for enzymatically labelling and click-labelling of V1 and V4 of HIV-1JR-FL (h, middle) and HIV-1BG505 Env (h, bottom).

A similar success rate was obtained for expression of 14 His-GST double tagged proteins examined by Western blotting using an anti-GST antibody.

Movie S1 shows typical migration sequences of wild type and THP-1 cells expressing the double tagged α2AAR-YFP/CFP in response to UK 14'304 and CCL2.

The first one detects full-length scFv using double Tag detection: an anti-HA-Tag as capture antibody (mAb 12CA5) and an AChE-labelled anti-His-Tag antibody was used as tracer.

In an anti-FLAG immunoprecipitate from the double tag strain, but not the single tag strains, both proteins were detected, and reciprocally, both SpSaf1-FLAG and SpDre4-GFP were detected in an anti-GFP immunoprecipitate (Figure 7A).