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The final verse, in which this double synthesis occurs, replicates in the minds of its readers the very experience it describes.
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The fragmented RNA was used for double strand synthesis followed by cDNA synthesis.
Ten µg DNase treated total RNA were used for a double cDNA synthesis reaction with oligo-dT primers and SuperScript™ III Reverse Transcriptase (Invitrogen).
Mature siRNAs are predominantly 24-nt long transcribed by RNA polymerase IV with subsequent double strand synthesis [ 4, 5].
New DNA sequencing techniques are currently being developed using single-molecule fluorescence-based detection of enzymatic double-strand synthesis.
Briefly, 10 μg total RNA for each sample was used to synthesize cDNA using a SuperScript double-strandedcDNA synthesis kit (Invitrogen) with oligo dT) primers for amplification.
Thereafter, the single-stranded cDNA was used as templates for double-stranded synthesis by PCR amplification using the primer designed according to the adapter sequence.
mRNA was purified with QIAGEN Oligotex mRNA Mini Kits (#70 022) using 25 µg of initial RNA, and used for double-stranded cDNA synthesis (SuperScript Double-Stranded cDNA synthesis #11917-010).
Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA).
Double stranded cDNA synthesis was carried out using 10 μg of total RNA and the components of the One Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, USA).
Architectural critic Philip Davies has noted that Viceroys House is a double magnificencea perfect synthesis of eastern and western architecture..
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