Exact(5)
In this paper, we introduced a double staining protocol using ruthenium red and TBO.
A sequential method for Amylase/CK-19 double staining was used according to the immunohistochemistry enzyme double staining protocol [ 31].
They were dried and post-stained using the standard double staining protocol of 4% (w/ v) uranyl acetate in 70% (w/ v) aqueous methanol 20 min followed by lead citrate for 2 min.
This is an underestimate of foci burden, as approximately a 10%% reduction in sense foci was noted using the double staining protocol, probably due to decreased sensitivity caused by the additional time at 80 °C during antisense probe hybridisation and washes.
H33342-induced flow changes did not affect results obtained using an in vivo double staining protocol provided that the interval between stain injections was greater than 5 min. Due to its transient effects on tumour perfusion, the stain caused radiobiological tumour hypoxia if injected immediately prior to X-irradiation.
Similar(55)
A sequential, double stain protocol was used for detecting co-expression of αSMA and OB-cadherin (visualized as orange-brown fluorescence).
The cell viability of PLL-MNP-labeled A549 cells was assessed by the FDA and PI double-staining protocol [39, 40].
Standard post-hybridization washes and double-stain protocols used an Affymetrix GeneChip Fluidics Station 400.
Standard post hybridization wash and double-stain protocols (EukGE-WS2v5_450) were used on an Affymetrix GeneChip Fluidics Station 450.
The standard wash and double-stain protocols were applied using a fluidics station (Affymetrix GeneChip Fluidics Station 450).
Standard post-hybridization wash and double-stain protocols (FS450_0001; GeneChip HWS hit; Affymetrix, Santa Clara, California, USA) were used on an Affymetrix GeneChip fluidics station 450.
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