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The anticancer activity of all derivatives were evaluated for colon cancer and breast cancer cell lines by the MTT assay and acridine orange/ethidium bromide double staining method.
HT-29 cells were incubated with inhibitors for a period of time and then measured via quantitative flow cytometry using Annexin V-PI double staining method.
MCF-7 cells were exposed to different concentrations of CA-4E for 24 h, and the FITC-Annexin V/PI double staining method for FCM was used to construct apoptotic cell scatter plots.
The annexin-V and PI double staining method was used.
To determine whether DHA (a representative omega-3 FA) also induced cell death, the annexin V/PI double staining method was used.
Apoptosis-mediated cell death of tumor cell was examined by a double staining method using FITC-labeled Annexin V/PI apoptosis detection kit (BD Bioscience, San Jose, CA) according to the manufacturer's instructions.
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The biocompatibility of the electrospun scaffolds and the viability of contacting cells were evaluated by 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) nuclear staining and by fluorescein diacetate (FDA) and propidium iodide (PI) double staining methods.
To investigate whether the drug can effectively induce apoptosis and necrosis of C. albicans cells, the apoptotic and necrotic features in CDA-AMB-NP-treated C. albicans cells were evaluated using annexin V-FITC and PI double-staining method.
We demonstrated that disc1 was expressed in the same region as olig2-positive cells using in situ hybridisation, but co-expression of disc1 in olig2-positive cells was not confirmed using double-staining methods.
In order to determine whether there is a link between OX1R and the apoptotic pathway in pancreatic islet cells, a double staining immunofluorescence method described above was used to identify OX1R and cleaved caspase 3 (pre-diluted; an apoptotic marker; Cell Signaling Technology, Inc., Beverly, MA, USA; Cat # 8120) simultaneously in pancreatic islets of OX−/− and C57BL/6 mice.
This study was designed to determine whether or not kallikrein has a potential to hydrolyze IGFBP-5 and their topographic proximity was investigated in rat brain using double immunohistochemical staining method.
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