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We also used double sites mutated probe as negative control, which exhibited diminished DNA-protein interaction band (lane 4).
In general, knockdown of PTPN18 by PTPN18-targeting siRNA did not perturb the phosphorylation states of the EGFR C-terminal Y, Y, pY, Y sites or the HER2 Y and Y1221/1222 double sites; however, the phosphorylation of the HER2 Y, Y and Y sites was significantly elevated in a time-dependent manner.
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No evidence of recombination was seen in 92 meiotic events, which would be indicated by a chromosome with either no NUMT or a doubled site.
Due to the pronounced chemical shift changes in purified MEKK3-PB1-I57tfmF MEKK3-PB1-I57tfmF MEKK3-PB1-I57tfmFe two sites were selected for double site-specific landling.
The double site-directed H306Q-H310E mutageneshowshow that G. graminis LOX activity is not causing the low yield of the G. graminis LOX.
Open image in new window Figure 2 One dimensional 19 F NMR spectra of MEKK3-PB1 or MEK5-PB1 in crude lysates with double site-specific tfmF incorporation.
To test whether the activity of the G. graminis LOX interferes with the correct synthesis and secretion of the protein a double site-directed H306Q-H310E mutagenesis was performed resulting in inactive A. nidulans GG-LOX mutants.
The second investigated URR bears a functional Cre1 double site [ 23].
Because KCTD15 has two predicted binding sites in its 3′-UTR, we performed molecular cloning for single site mutations to obtain vectors named MUT1-KCTD15, MUT2-KCTD15, and double site mutation vectors named MUT1,2-KCTD15 MUT1,2-KCTD15
Therefore, the following modifications were introduced (protein sequences are indicated by UNIPROT accession numbers): The first phosphorylation site of P02646 is actually a double site that lies at positions 22/23 instead of position 20 [ 59, 60].
Given the possibility of different but highly related HPV E2C domains, we compared binding of E2C domains from HPV11, HPV16, and HPV18 (E2C-16, E2C-18, and E2C-18) to individual single sites and the double site from the HPV16 genome.
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