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These experiments were carried out at 45,000 r.p.m. with 400-μL samples at 37 μM in 5 mM sodium phosphate buffer (pH 6.3) loaded into double sector cells.
Twelve mm path length double sector cells were used for all samples.
Buffer (400 µl) and sample solutions (380 µl) were loaded into the double sector centerpiece separately and established in a Beckman An-50 Ti rotor.
Sedimentation velocity experiments were performed in a Beckman XL-A analytical ultracentrifuge using a double sector charcoal-Epon cell at 20°C and 40,000 rpm.
Sedimentation velocity experiments were performed in a Beckman XL-I analytical ultracentrifuge using a double sector charcoal-Epon cell at 20°C and 42000 rpm.
400µl protein was loaded into a double sector Epon cell with sapphire windows and centrifuged at 50,000rpm (182 000rcf at cell centre and 201 600rcf at cell bottom) at 20°C using an An-50Ti rotor (Beckman Coulter).
Similar(40)
The practical use of the method is demonstrated with double-sector and single-sector sedimentation velocity experiments, and with analytical electrophoresis experiments.
Sample was loaded into a conventional double-sector quartz cell and mounted in a Beckman four-hole An-60 Ti rotor.
A sedimentation velocity experiment was performed by using a Beckman/Coulter XL-I analytical ultracentrifuge at 60,000 rpm and 4 °C using absorbance detection and double-sector cells loaded with approximately 15 mM RBUP (Zhang et al. 2014).
analytical ultracentrifuge at rotor speeds of 19000, 22000, and 29000 rpm in double-sector charcoal-filled Epon centerpiece.
For the sedimentation velocity experiment the loading volume of 400 µl was identical for the reference and sample chambers of the double-sector centrepiece.
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