Sentence examples for double restrictions from inspiring English sources
Therefore, faced with the double restrictions, the entrepreneurs of the SMEs who held market power and personalized social networks for purchasing, i.e., subcontractors,11 began to adjust the capital settlement period within upstream contracts in order to tackle the problem of capital shortage.
The plasmid complement of transformants was verified by PCR and double restriction digestion.
pBAD-dsbA was purified from transformants and its integrity verified by double restriction digestion and DNA sequencing.
pSU18-mcr-1 was transformed into E. coli TOP10 (Invitrogen), purified and its integrity verified by double restriction digestion and DNA sequencing.
The expression vector pET-32a-EG was verified with colony PCR and double restriction endonuclease digestion.
The recombinant plasmid was then successfully verified using double restriction digest and PCR.
Thus, the SMEs were under the double restriction of a shortage of regular capital supply channels and the high cost of irregular capital supply (Zhou 2007).
Following the double restriction digestion, the genomic DNA fragment was cloned into a binary vector, pZH2B-GUS (Kuroda et al. 2010), with a pre-existing insertion of the beta-glucoronidase (GUS) gene.
A double restriction digest was performed on both the plasmid pBBR1MCS-2 (see Table 2) and the inserts, using Kpn1 and Xba1 for the formation of pER1, pER2, pER4, or Sal1 and Xba1 for the formation of pER3.
Yet to have any hope of achieving this we would need a double restriction: the only preferences to be considered must be now-for-now or then-for-then, and they must be fully internal in relating only to conscious states of the subject indeed, more precisely, to features of those states of which the agent is conscious, or, for short, what it is like to be in those states.
I'm hopelessly nostalgic and thankful that today's technology did not exist, leading me to be on a lifetime of permanent double restriction with no phone or TV privileges.
